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Equal amounts of protein (30 μg) from each sample were separated by SDS-PAGE and then transferred onto PVDF membranes (Millipore, United States)

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This inhibitory activity was likely mediated through the upregulation of caspase-3, PARP, and the Bax/Bcl-2 ratio, thereby inducing apoptosis in HT29 cells and subsequently suppressing the progression of CRC ( 4.2 TCM formulations target the Wnt/β-catenin signaling pathway to treat CRC TCM formulations could protect the intestinal barrier integrity in CRC cells