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DSIP 5mg | Swan Peptides

Step 1 tert-Butyl 4-(6-nitropyridin-3-yl-2,4,5-d3)pipera/.ine-l -carboxylate Step 2 tert-butyl 4-(6-aminopyridin-3-yl-2,4,5-d;0piperazine-l -carboxylate Step 3 ter/-Butyl 4-(6-((7-cyclopentyl-6-(dimethylcarbamoyl)-7H-pyrroloL2,3- d Step 4 7-Cyclopentyl-N,N-dimethyl-2-((5-(pipera/.in-l-yl)pyridin-2-yl-3,4,6- d3)amino)-7H-pyrrolo Example 3 was prepared from 2-chloro-7-cyclopentyl-N, N-dimethyl-7H-pynolo[2,3-d Example 4 was prepared from 2-chloro-7-cyclopentyl-N, Nslimemyl-7H-pyrrolo Step 1 tert-Bulyl 4-(pyridin-3-yl-d 4 )piperazine-l-carboxylate (commercially available, 500 mg, 3.09 mmol), piperazine-l-carboxylic acid ten-butyl ester (690 mg, 3.70 mmol), PdC12(dppf) (1 13 mg, 0.15 mmol), and potassium tert butoxide (800 mg, 7.1 mmol) was degassed and stirred at 100°C for 14 hours under a nitrogen atmosphere

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RNA isolation and quantitative real-time PCR Skeletal muscle samples from the gastrocnemius muscles were homogenized in 1.5 mL Eppendorf ® tubes with tungsten carbide and micro-glass beads using a TissueLyser LT system (Qiagen, USA) pre-cooled to − 80 °C